Published May 1998
by S Karger Pub .
Written in English
|The Physical Object|
|Number of Pages||140|
Nucleic acid amplification is a pivotal process in biotechnology and molecular biology and has been widely used in research, medicine, agriculture and forensics. Polymerase chain reaction (PCR) was the first nucleic acid amplification method developed and until now has been the method of choice since its invention by Mullis. PCR is the. Brian Castillo, Amer Wahed, in Transfusion Medicine for Pathologists, Methodology Used for Infectious Disease Screen. Nucleic acid testing (NAT) either by polymerase chain reaction (PCR) or transcription-mediated amplification is used to test for HIV-1, HCV, HBV, WNV, and Zika virus. HIV NAT detects HIV-1 RNA which further reduces the window period of infection and positive test. Nucleic acid amplification technology methods used in blood donor screening Article Literature Review in Transfusion Medicine 12(4) September with Reads How we measure 'reads'. 1. Introduction. A variety of nucleic acid amplification techniques were developed in the mid to late 's. These include the polymerase chain reaction (PCR) (Mullis and Faloona, ), ligation-mediated amplification (Wu and Wallace, ) and transcription-based amplification (Kwoh et al., ).Since then, these techniques have been refined and alternative approaches have been developed Cited by:
Background The use of nucleic acid amplification tests of “minipools” of 16 samples to screen blood donors for West Nile virus RNA began in July We report the yield and characteristics Cited by: Detection. Infection with the HDV is recognized by the finding of the homologous antibody (anti-HD) in g for the viral genome (HDV RNA) is , the 1 st World Health Organization International Standard of HDV RNA for nucleic acid amplification techniques (NAT)-based assays was developed.. The underlying HBV infection required to support the HDV is critical to determine. Blood bank 5th Edition By Harmening 1. which intro-duces the student to nucleic acid techniques and theory that govern molecular genetics in determining compati-bility between donor and recipient, production of recom-binant proteins such as growth factors utilized in certain apheresis procedures, and detection of transfusion-trans-mitted. Nucleic acid amplification tests and, in particular, reverse transcriptase (RT)-PCR assays are ideal when considering improved sensitivity, worker biosafety protection, and enhanced viral.
When researchers can detect pathogens from cells or tissues reliably, they’re better able to study responses to infection or responses to an experimental treatment against the pathogen. Recently, older culture-based methods have been outpaced by newer, nucleic acid-based techniques, which offer greater convenience, speed, sensitivity and specificity. This test amplifies the nucleic acid in a virus more than a million-fold, allowing early detection of minute quantities of virus in the blood. Blood donors to the National Institutes of Health's Department of Transfusion Medicine (blood bank) will have their blood screened with transcription mediated amplification, a type of NAT test. Development & Implementation of Nucleic Acid-based Tests. A range of laboratory-developed and commercial RT-PCR assays using diverse . variability enhance implementation of nucleic acid testing (NAT). Nucleic Acid Testing (NAT) for detection of human immunodeficiency virus (HIV), hepatitis C virus (HCV) and hepatitis B virus (HBV) has become a routine part of blood donor infectious screening. Hepatitis B Virus (HBV) is one of several viruses known to cause viral hepatitis.